Biological and methodological implications of prostaglandin involvement in mouse brain lipid peroxidation measurements
Identifieur interne : 004B22 ( Main/Exploration ); précédent : 004B21; suivant : 004B23Biological and methodological implications of prostaglandin involvement in mouse brain lipid peroxidation measurements
Auteurs : Ranjan Bose [Canada] ; Garnette R. Sutherland [Canada] ; Carl Pinsky [Canada]Source :
- Neurochemical Research [ 0364-3190 ] ; 1989-03-01.
Abstract
Abstract: Enhanced cyclooxygenase-mediated prostaglandin (PG) turnover occurring during sacrifice and biochemical processing of tissues also generates malondialdehyde (MDA), a product of lipid peroxidation (LPO). Studies reporting on LPO estimated by thiobarbituric acid reactive substances (TBARS) have failed to consider such artefactual increases. This study reports the relative proportion of PG metabolism-derived MDA (PG-MDA) in mouse brain regions during the TBARS assay. The cyclooxygenase inhibitor indomethacin significantly lowered MDA in fronto-parietal cortex and corpus striatum. Indomethacin (50–800 μg/ml, in vitro) increased estimated TBARS in whole brain. Such enhancement was absent when indomethacin (20–80 μg/sample) was added to the MDA standard curve, reflecting its interaction with TBARS other than MDA. PG-MDA contributes as much as 15% to the total estimated value of MDA in fronto-parietal cortex and corpus striatum and must be corrected for in LPO studies.
Url:
DOI: 10.1007/BF00971313
Affiliations:
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Studies reporting on LPO estimated by thiobarbituric acid reactive substances (TBARS) have failed to consider such artefactual increases. This study reports the relative proportion of PG metabolism-derived MDA (PG-MDA) in mouse brain regions during the TBARS assay. The cyclooxygenase inhibitor indomethacin significantly lowered MDA in fronto-parietal cortex and corpus striatum. Indomethacin (50–800 μg/ml, in vitro) increased estimated TBARS in whole brain. Such enhancement was absent when indomethacin (20–80 μg/sample) was added to the MDA standard curve, reflecting its interaction with TBARS other than MDA. PG-MDA contributes as much as 15% to the total estimated value of MDA in fronto-parietal cortex and corpus striatum and must be corrected for in LPO studies.</div></front></TEI><affiliations><list><country><li>Canada</li></country><region><li>Manitoba</li></region><settlement><li>Winnipeg</li></settlement><orgName><li>Université du Manitoba</li></orgName></list><tree><country name="Canada"><region name="Manitoba"><name sortKey="Bose, Ranjan" sort="Bose, Ranjan" uniqKey="Bose R" first="Ranjan" last="Bose">Ranjan Bose</name></region><name sortKey="Pinsky, Carl" sort="Pinsky, Carl" uniqKey="Pinsky C" first="Carl" last="Pinsky">Carl Pinsky</name><name sortKey="Sutherland, Garnette R" sort="Sutherland, Garnette R" uniqKey="Sutherland G" first="Garnette R." last="Sutherland">Garnette R. Sutherland</name><name sortKey="Sutherland, Garnette R" sort="Sutherland, Garnette R" uniqKey="Sutherland G" first="Garnette R." last="Sutherland">Garnette R. 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